Escherichia coliproteome analysis using the gene‐protein database

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Abstract
The gene‐protein database ofEscherichia coliis a collection of data, largely generated from the separation of complex mixtures of cellular proteins on two‐dimensional (2‐D) polyacrylamide gel electrophoresis. The database currently contains about 1600 protein spots. The data are comprised of both identification information for many of these proteins and data on how the level or synthesis rates of proteins vary under different growth conditions. Three projects are underway to further elucidate theE. coliproteome including a project to localize on 2‐D gels all of the open reading framed encoded by theE. colichromosome, a project to determine the condition(s) under which each open reading frame is expressed and a project to determine the abundance and location of each protein in the cell. Applications for proteome databases for cell modeling are discussed and examples of applications in therapeutic drug discovery are given.