Variations du taux en composés sulfhydrilés acidosolubles de tissus cultivés in vitro

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Abstract
Variations of the Level of the Nitrogen and Thiol Groups in Tissues Culture.—Distribution of nitrogen and thiol compounds was analysed in the fragments of carrot roots cultivated in vitro. A typical gradient of –SH groups was observed: high level in the callus, low level in the base of the fragment (in contact with the medium). In discontinuous light, callus—which have a great amount of chlorophylls—contain less sulfhydric compounds than in continuous dark. With increasing age of the tissues, the concentration of the endogenous –SH groups was decreasing. The probable biochemical relations between –SH level, auxin content and auxin‐oxidase activity are discussed.