Evidence for the transcriptional control of nitrate reductase in Candida nitratophila from in vitro translation studies

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Abstract
In vivo labelling and in vitro translation studies were used to study the regulation of the synthesis of nitrate reductase in the yeast Candida nitratophila. These studies showed that synthesis of the enzyme subunit took place when ammonium-grown cells were nitrogen-starved and this was stimulated by subsequent addition of nitrate. Ammonium-grown cultures did not contain mRNA that could be translated into the nitrate reductase subunit in an in vitro system. Nitrate reductase mRNA could be extracted from nitrogen-starved and nitrate cultures. Synthesis of the enzyme is apparently controlled at the level of transcription in this yeast.