Effects of Recombinant Apolipoprotein A-I Milano on Aortic Atherosclerosis in Apolipoprotein E–Deficient Mice
- 3 March 1998
- journal article
- other
- Published by Wolters Kluwer Health in Circulation
- Vol. 97 (8) , 780-785
- https://doi.org/10.1161/01.cir.97.8.780
Abstract
Background—We previously reported marked inhibitory effects of recombinant apolipoprotein (apo) A-IMilano/phospholipid complex (A-IMilano/PC) on neointimal lesions in balloon-injured iliofemoral arteries of hypercholesterolemic rabbits. In this study, we tested the hypothesis that apo A-IMilano/PC would inhibit aortic atherosclerosis in apo E–deficient mice. Methods and Results—Thirty-five apo E–deficient mice fed a high-cholesterol diet were included in the study. Control mice were killed at 20 (n=8) or 25 (n=7) weeks. Treated mice received 18 injections of either 40 mg/kg apo A-IMilano/PC (n=15) or PC only (n=5) intravenously every other day from 20 weeks until death at 25 weeks. Aortic atherosclerosis was identified with Sudan IV staining. Lipid and macrophage contents of the aortic sinus plaques were measured after oil-red O and Mac-1 antibody staining, respectively, and quantified with computed morphometry. In control mice, from 20 to 25 weeks, aortic atherosclerosis increased by 59% (11±1% versus 17...Keywords
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