In vitro evidence that the coat protein is the programming factor in alfalfa mosaic virus‐induced RNA synthesis
- 14 September 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 221 (2) , 337-342
- https://doi.org/10.1016/0014-5793(87)80952-3
Abstract
Viral RNA‐dependent RNA polymerase preparations were isolated from cowpea mesophyll protoplasts 26 h after inoculation with the complete genome (RNAs 1, 2 and 3) and the incomplete genome (RNAs 1 and 2) of alfalfa mosaic virus. Although in incompletely inoculated protoplasts the ratio minus‐/plus‐strand RNA synthesis is much higher than in completely inoculated ones [(1983) Virology 124, 75–85)], both enzyme preparations produced largely plus‐strand RNA on their endogenous templates. When made template‐dependent both were strongly inhibited by small amounts of coat protein. The experiments show that among the two proteins encoded by RNA 3 only the (parental) coat protein can be responsible for the programming of the viral RNA polymerase to plus‐strand synthesis.Keywords
This publication has 11 references indexed in Scilit:
- Coat protein blocks the in vitro transcription of the virion RNAs of alfalfa mosaic virusFEBS Letters, 1986
- Vira RNA synthesis by a particulate fraction from cucumber seedlings infected with cucumber mosaic virusVirology, 1985
- Human leukocyte interferon does not inhibit alfalfa mosaic virus in protoplasts or tobacco tissueVirology, 1985
- Virus protein synthesis in alfalfa mosaic virus infected alfalfa protoplastsVirology, 1983
- Altered balance of the synthesis of plus- and minus-strand RNAs induced by RNAs 1 and 2 of alfalfa mosaic virus in the absence of RNA 3Virology, 1983
- Evidence that alfalfa mosaic virus infection starts with three RNA-protein complexesVirology, 1981
- Molecular weights of particles and RNAs of alfalfa mosaic virus. Number of subunits in protein capsidsBiochemistry, 1977
- Factors Influencing the Infection of Cowpea Mesophyll Protoplasts by Alfalfa Mosaic VirusJournal of General Virology, 1977
- Studies on sequence homology between the RNA's of alfalfa mosaic virusVirology, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970