Peptide Nucleic Acid-Mediated Competitive PCR Clamping for Detection of Rifampin-Resistant Mycobacterium tuberculosis
Open Access
- 1 October 2004
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 48 (10) , 4023-4026
- https://doi.org/10.1128/aac.48.10.4023-4026.2004
Abstract
Peptide nucleic acid-mediated competitive PCR clamping, which can selectively amplify mutant alleles, was developed to detect mutations in four codons (513, 516, 526, and 531) of the rpoB gene in Mycobacterium tuberculosis strains. This simple method successfully identified the mutations in all 40 of the M. tuberculosis strains tested.Keywords
This publication has 17 references indexed in Scilit:
- [Fluctuation in the ratio between drug-resistant and -susceptible M. tuberculosis bacilli which were isolated from patients with prolonged anti-tuberculosis treatment].2003
- Novel Polymorphic Region of the rpoB Gene Containing Mycobacterium Species-Specific Sequences and Its Use in Identification of MycobacteriaJournal of Clinical Microbiology, 2003
- Peptide Nucleic Acid Probe Detection of Mutations in Mycobacterium tuberculosis Genes Associated with Drug ResistanceBioTechniques, 2002
- Detection of Rifampin Resistance in Mycobacterium tuberculosis in a Single Tube with Molecular BeaconsJournal of Clinical Microbiology, 2001
- Comparison of two nonradioactive, single-strand conformation polymorphism electrophoretic methods for identification of rpoB mutations in rifampin-resistant isolates of Mycobacterium tuberculosis*, **Molecular Diagnosis, 1998
- Molecular genetic basis of antimicrobial agent resistance inMycobacterium tuberculosis: 1998 updateTubercle and Lung Disease, 1998
- Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assayTubercle and Lung Disease, 1995
- The rpoB gene of Mycobacterium tuberculosisAntimicrobial Agents and Chemotherapy, 1994
- Direct, automated detection of rifampin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysisAntimicrobial Agents and Chemotherapy, 1993
- Detection of rifampicin-resistance mutations in Mycobacterium tuberculosisThe Lancet, 1993