Purification and some properties of cephalosporin N, a new penicillin

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Abstract
Cephalosporin N is an acidic antibiotic produced by a strain of Cephalosporium. It is inactivated by the enzyme penicillinase but differs strikingly from the common penicillins in its antibacterial activity and hydrophilic character. Cephalosporin N was purified by chromatography on charcoal and alumina and by countercurrent distribution in a phenol-water system containing 2:4:6-trimethylpyridine as a carrier. The resulting product appears to be at least 75% pure. The acid-base properties and solubility of cephalosporin N, together with its chemical reactivity, suggest that it is a penicillin containing an aminodicarboxylic acid in the side chain.