Characterization of Murine Liver‐Derived Inhibitory Protein

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Abstract
Murine liver‐derived inhibitory protein (LIP) capable of inhibiting human lymphocyte proliferation was highly purified from liver extract. Its molecular weight determined by gel filtration and SDS‐PAGE was 105,000 and 38,400 respectively. LIP moved electrophoretically at the gammaglobulin region. Its activity in inhibiting lymphocyte proliferation was temperature‐stable up to 60° C, and pH‐stable between 4 and 11. It was not cytotoxic to lymphocytes as shown in 51 Crrelease experiments. The purified LIP possessed arginase activity.