A Rapid Screen Test for In Vitro Susceptibility of Clinical Herpes Simplex Virus Isolates

Abstract
A rapid screening test for antiviral susceptibility to acyclovir and foscarnet was evaluated in 320 herpes simplex virus (HSV) isolates from 197 patients. Ofthe isolates tested (16% HSV-1, 84% HSV-2), 60% were resistant in vitro to acyclovir and 5% were resistant to foscarnet using the plaque reduction assay. The rapid screening test, requiring only 3 days to yield a preliminary susceptibility result, was highly associated with the results of the plaque reduction assay for acyclovir and foscarnet (P ⩽.001 for each) and with the clinical response to antiviral therapy (P < .001, P = .001, respectively).

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