Intermediate states in the movement of transfer RNA in the ribosome
- 1 November 1989
- journal article
- Published by Springer Nature in Nature
- Vol. 342 (6246) , 142-148
- https://doi.org/10.1038/342142a0
Abstract
Direct chemical 'footprinting' shows that translocation of transfer RNA occurs in two discrete steps. During the first step, which occurs spontaneously after the formation of the peptide bond, the acceptor end of tRNA moves relative to the large ribosomal subunit resulting in 'hybrid states' of binding. During the second step, which is promoted by elongation factor EF-G, the anticodon end of tRNA, along with the messenger RNA, moves relative to the small ribosomal subunit.Keywords
This publication has 33 references indexed in Scilit:
- Specific recognition of the 3′-terminal adenosine of tRNAPhe in the exit site of Escherichia coli ribosomesJournal of Molecular Biology, 1988
- Rapid chemical probing of conformation in 16 S ribosomal RNA and 30 S ribosomal subunits using primer extensionJournal of Molecular Biology, 1986
- Comparison of ribosomal entry and acceptor transfer ribonucleic acid binding sites on Escherichia coli 70S ribosomes. Fluorescence energy transfer measurements from Phe-tRNAPhe to the 3' end of 16S ribonucleic acidBiochemistry, 1983
- Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomesFEBS Letters, 1983
- Interaction of siomycin with the acceptor site of Escherichia coli ribosomesJournal of Molecular Biology, 1972
- The Binding of Purified Phe-tRNA and Peptidyl-tRNAPhe to Escherichia coli RibosomesEuropean Journal of Biochemistry, 1971
- Movement of the ribosome along the messenger ribonucleic acid during protein synthesisBiochemistry, 1971
- Stimulation of “non‐enzymic” translocation in ribosomes by p‐chloromercuribenzoateFEBS Letters, 1971
- Binding of transfer ribonucleic acid to ribosomes engaged in protein synthesis: Number and properties of ribosomal binding sitesJournal of Molecular Biology, 1965
- The puromycin reaction and its relation to protein synthesisJournal of Molecular Biology, 1964