During B-Z Transition There is No Large Scale Breakage of Watson-Crick Base Pairs A Direct Demonstration Using 500 MHz1H NMR Spectroscopy
- 1 October 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 1 (1) , 59-81
- https://doi.org/10.1080/07391102.1983.10507426
Abstract
Monitoring of the Watson-Crick GNH1 proton in poly(dG-dC)-poly(dG-dC) at 500 MHz in 90% H20:10% D2o at 30° C as a function of NaCl concentration (1.5 to 3.6 M), demonstrates that the bases retain Watson-Crick pairing throughout the transition. This observation unequivocally demonstrates that during the B-Z transition there is no large scale and detectable base pair opening and that macroscopically the phenomenon can be described as a direct helix to helix transition. We present frame by frame, an energetically sound stereodynamical trajectory for this transfiguration from right-handed B-DNA to left-handed Z-DNA.This publication has 9 references indexed in Scilit:
- A condensed form of (dG-dC)n.cntdot.(dG-dC)n as an intermediate between the B- and Z- conformations induced by sodium acetateBiochemistry, 1983
- Physical evidence for a domain structure in Escherichia coli 5 S RNAFEBS Letters, 1983
- Theoretical Probes of DNA Conformation Examining the B->Z Conformational TransitionCold Spring Harbor Symposia on Quantitative Biology, 1983
- Plasticity of the DNA double helixJournal of the American Chemical Society, 1981
- The high salt form of poly(dG-dC)·poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutionsNucleic Acids Research, 1981
- Left-handed deoxyribonucleic acid double helix in solutionBiochemistry, 1981
- Structural motifs of the nucleotidyl unit and the handedness of polynucleotide helicesInternational Journal of Quantum Chemistry, 1981
- Molecular structure of a left-handed double helical DNA fragment at atomic resolutionNature, 1979
- Salt-induced co-operative conformational change of a synthetic DNA: Equilibrium and kinetic studies with poly(dG-dC)Journal of Molecular Biology, 1972