Application of Fast LC to Pharmaceutical Analysis: Dopamine * HCI

Abstract
A reversed-phase HPLC method for the determination of the dopamine hydrochloride content of pharmaceutical intravenous solutions is developed using short (3-cm) columns packed with 3-µm particles. Total analysis time for the stability indicating assay, which is capable of separating the analyte from all its common degradates and formulation matrix components, is 2 min. The assay is equivalent in performance (accuracy, precision, separation behavior, ruggedness) to a standard method recommended by the United States Pharmacopeia (USP). Common components of conventional HPLC systems are evaluated with respect to their utility for this application of fast LC.

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