Plaque assay and cloning of scrub typhus rickettsiae in irradiated L-929 cells
- 1 July 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 6 (1) , 76-80
- https://doi.org/10.1128/jcm.6.1.76-80.1977
Abstract
.gamma.-irradiated L-929 cells support plaque formation by 3 strains of Rickettsia tsutsugamushi and representative species of the spotted fever and typhus group rickettsiae. Sensitivity of the plaque assay for detection of viable scrub thyphus rickettsiae was similar to that achieved with i.p. inoculation of random-bred mice. The concentration of irradiated cells and the temperature and length of incubation all affected plaque size. A technique combining terminal dilution and plaque purification was used to obtain clones of 3 strains of scrub typhus rickettsiae.This publication has 11 references indexed in Scilit:
- Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells.1974
- Plaque assay of rickettsiae in a mammalian cell line.1974
- Metabolism of Richettsia tsutsugamushi and Rickettsia rickettsi in irradiated host cells.1973
- Effects of Various Suspending Media on Plaque Formation by Rickettsiae in Tissue CultureInfection and Immunity, 1972
- Metabolism of Rickettsia typhi and Rickettsia akari in Irradiated L CellsInfection and Immunity, 1972
- Studies of the Rickettsial Plaque Assay TechniqueInfection and Immunity, 1972
- Plaque assay for Q fever and scrub typhus rickettsiae.1970
- Plaque Assay System for Several Species of RickettsiaJournal of Bacteriology, 1969
- IMMUNIZATION AGAINST SCRUB TYPHUSAmerican Journal of Epidemiology, 1951
- THE INFLUENCE OF CERTAIN SALTS, AMINO ACIDS, SUGARS, AND PROTEINS ON THE STABILITY OF RICKETTSIAEJournal of Bacteriology, 1950