Automatic Description of the Venation of Mosquito Wings from Digitized Images

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Abstract
Methods are described for the automatic measurement of morphological features of mosquito wings (wing outline and venation) from TV images of specimens mounted on microscope slides. The digitized images were preprocessed to locate the "ridge points" in the gray-scale image surface and the digitized images were thinned so that veins could be represented by curves only a single pixel wide. The image was then segmented into the wing outline and the 10 longitudinal wing veins. Polynomial functions were fitted to each vein. The coordinates of the end points of each vein and the coefficients of the polynomials could then be used as descriptors of each wing for subsequent multivariate analyses. Comparisons are made with other algorithms and the results are shown.