The effects of kinetin and contaminating bacteria on the incorporation of 32P-orthophosphate into various fractions of nucleic acid extracted from radish leaves
- 1 January 1968
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 81 (1) , 88-96
- https://doi.org/10.1007/bf00385518
Abstract
By comparing 32P-orthophosphate incorporation into nucleic acid extracts of sterile and non-sterile radish leaf discs, it was shown that contaminating bacteria cause a 2–4 fold increase in the rate of precursor incorporation and alter the pattern of label distribution after fractionation of the extracts by sedimentation through sucrose gradients or chromatography on MAK1 columns. Using sterile senescing radish leaf discs, a stimulation of 32P-orthophosphate incorporation into various fractions of nucleic acid was observed as a result of kinetin treatment.Keywords
This publication has 19 references indexed in Scilit:
- Cytokinins in the Soluble RNA of Plant TissuesScience, 1967
- A cytokinin in soluble RNA from a higher plantLife Sciences, 1967
- Nucleic Acid Synthesis in SeedlingsNature, 1967
- Cytokinin Activity: Localization in Transfer RNA PreparationsScience, 1966
- Hybridization of messenger RNA with DNA from plantsBiochemical and Biophysical Research Communications, 1966
- Struktur eines ungewöhnlichen Nucleosids aus serin‐spezifischer Transfer‐RibonucleinsäureAngewandte Chemie, 1966
- Nucleotidsequenzen zweier serinspezifischer Transfer‐RibonucleinsäurenAngewandte Chemie, 1966
- Demonstration and characterization of a DNA-like RNA in excised plant tissueJournal of Molecular Biology, 1965
- Patterns of messenger RNA synthesis in mammalian tissues: Analysis by counter-current distributionJournal of Molecular Biology, 1964
- Isolation and purification of undegraded ribonucleic acidsBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964