Rna Polymerase Activities in Liver and Brain Tissue of Aging Mice
- 1 May 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Gerontology
- Vol. 33 (3) , 323-328
- https://doi.org/10.1093/geronj/33.3.323
Abstract
RNA polymerase activity has been measured in liver and brain of C57B1/6J mice to determine if a change in enzyme activity can be correlated with decreasing survivorship of the animals. The RNA polymerases in tissue homogenates were solubilized by treatment with a buffer of high ionic strength and resolved by DEAE-Sephadex chromatography. Enzyme activity was quantitated by measuring the incorporation of [3H] UMP into RNA using heat denatured calf thymus DNA as the template. Statistically significant differences in polymerase activities were not observed in liver tissue from 18-, 25-, and 29-month-old animals or in brain tissue from 23- to 31-month-old animals. These age groups span the period of most rapid decrease in survivorship in our colony of mice (from 93% to 16%). The evidence indicates that changes in liver or brain RNA polymerase activities are not correlated with the rapid decrease in survivorship of these animals.Keywords
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