In vitro regeneration of innervated forelimb deplants of Ambystoma larvae

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Abstract
The small cell aggregate of a young innervated [A. opacum and A. maculatum] larval forelimb blastema was capable of undergoing growth and differentiation in vitro after explantation. The noninnervated forelimb blastema controls showed little or no development in culture. There also was a nerve requirement in vitro.