GROWTH ENHANCEMENT OF THE REITER TREPONEME BY FATTY ACIDS,

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Abstract
Quantitative measurements of cell crop were performed on the Reiter strain of Treponema pallidum grown in spirolate broth (BBL) plus 10% inactivated sheep serum with and without certain lipoidal supplements. A correlation of results obtainable by direct counting, protein N determination, cellular weight, and turbidimetric techniques was made. The cell crop was increased 2- to 3-fold when the medium was supplemented with TEM 4T (the diacetyl tartaric acid ester of tallow monoglycerides) at a concentration of 0.20 to 0.25 mg/ml of the medium. The TEM 4T could be replaced by a mixture of palmitic, stearic, and oleic acids, in equal amounts, and at a total fatty acid concentration of 0.20 mg/ml.