A monoclonal antibody recognizing a determinant common to HLA-A3 and All
- 1 February 1982
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 19 (2) , 121-128
- https://doi.org/10.1111/j.1399-0039.1982.tb01427.x
Abstract
Spleen cells from a mouse immunized with an AML cell expressing HLA-A3 produced a hybridoma secreting an anti-HLA—A3, All monoclonal antibody, 26D3. By complement-dependent cytotoxicity at dilutions to 1:104 the ascites antibody lysed 13/13 A3. 15/15 A11, and 8 other lymphocytes from a panel of 98 donors. The 26 D3 immunoprecipitated a molecule consisting of subunits of 44,000 and 12,000 daltons. Monoclonality of the antibody was demonstrated by isoelectric focusing and protein A affinity chromatography.This publication has 12 references indexed in Scilit:
- Monoclonal antibodies defining serologically distinct HLA-D/DR related Ia-like antigens in manHuman Immunology, 1981
- A monoclonal antibody that recognizes an antigenic determinant shared by HLA A2 and B17Human Immunology, 1980
- Monoclonal Antibodies for Analysis of the HLA SystemImmunological Reviews, 1979
- Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2Nature, 1978
- Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycolurilBiochemistry, 1978
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Somatic cell hybrids between human lymphoma lines. III. Surface markersInternational Journal of Cancer, 1977
- RABBIT ANTISERA TO HL-A9 ISOLATED FROM NORMAL SERUMTransplantation, 1974
- Polypeptides of the tail fibres of bacteriophage T4Journal of Molecular Biology, 1971
- Microdroplet Assay of Human Serum CytotoxinsNature, 1964