Selective cytotoxic activity of immunotoxins composed of a monoclonal anti-Thy 1.1 antibody and the ribosome-inactivating proteins bryodin and momordin

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Abstract
The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charamtia were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKR-A cells by 50% at a concentration of 1-4 .times. 10-11 M as compared with 1 .times. 10-9 M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma E14 cells which lack the Thy 1.1 antigen, at concentration up to 3 .times. 10-8 M. Further bryodin and momordin immunotoxins made from an antibody (R10) of irrelevant specificity were without effect on AKR-A cells.

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