A comparison of the substrate specificity of MAPKAP kinase‐2 and MAPKAP kinase‐3 and their activation by cytokines and cellular stress
Open Access
- 2 September 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 392 (3) , 209-214
- https://doi.org/10.1016/0014-5793(96)00816-2
Abstract
MAPKAP kinase‐2 and MAPKAP kinase‐3 were both activated in response to cellular stress, interleukin‐1 and tumour necrosis factor in KB and HeLa cells, and with identical kinetics. Activation of MAPKAP kinase‐3, like MAPKAP kinase‐2, was prevented by SB 203580, a specific inhibitor of SAPK‐2, the upstream activator of MAPKAP kinase‐2. MAPKAP kinase‐3 and MAPKAP kinase‐2 phosphorylated peptide substrates with similar kinetic constants and phosphorylated the same serine residues in HSP27 at the same relative rates. These results establish that MAPKAP kinase‐3 lies ‘downstream’ of SAPK‐2 and that it is likely to have overlapping or identical substrates to MAPKAP kinase‐2 in vivo.Keywords
This publication has 22 references indexed in Scilit:
- SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin‐1Published by Wiley ,2000
- p38/RK is essential for stress-induced nuclear responses: JNK/SAPKs and c-Jun/ATF-2 phosphorylation are insufficientCurrent Biology, 1996
- Defective thymocyte proliferation and IL-2 production in transgenic mice expressing a dominant-negative form of CREBNature, 1996
- Identification of a Dual Specificity Kinase That Activates the Jun Kinases and p38-Mpk2Science, 1995
- Pro-inflammatory Cytokines and Environmental Stress Cause p38 Mitogen-activated Protein Kinase Activation by Dual Phosphorylation on Tyrosine and ThreonineJournal of Biological Chemistry, 1995
- A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteinsPublished by Elsevier ,1994
- Interleukin-1 activates a novel protein kinase cascade that results in the phosphorylation of hsp27Cell, 1994
- A MAP Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian CellsScience, 1994
- Nerve growth factor activates a Ras-dependent protein kinase that stimulates c-fos transcription via phosphorylation of CREBCell, 1994
- Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteinsFEBS Letters, 1992