Biosynthesis of Gramicidin S with the Aid of Dipeptides by Gramicidin S Synthetase
- 1 July 1976
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 66 (3) , 623-626
- https://doi.org/10.1111/j.1432-1033.1976.tb10590.x
Abstract
Dipeptides L-phenylalanyl-proline, D-phenylalanyl-proline, prolyl-valine, valyl-lysine, lysyl-leucine and leucyl-phenylalanine, derived from the sequence of gramicidin S, are substrates of the gramicidin S synthetase. When any of these dipeptides are used to replace the two corresponding amino acids in the reaction assay, cyclodecapeptide antibiotic synthesis occurs, and requires the whole multienzyme system. Active esters, like the thiophenyl and p-nitrophenyl esters of D-phenylalanyl-proline are unable to promote gramicidin S biosynthesis with the gramicidin S synthetase system or with the heavy enzyme alone.Keywords
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