Molecular cloning of an ice nucleation gene from Erwinia ananas and its expression in Escherichia coli

Publisher Website
Google Scholar
Abstract
An approximately 7 kbp genomic DNA fragment was cloned from an ice nucleation-active (ina) strain of Erwinia ananas and defined as to its restriction enzyme site. When the DNA fragment was introduced into E. coli MM294, a potent ice nucleation activity was expressed. Both 0.7 kbp truncation from the 5′-end and 1.7 kbp truncation from the 3′-end were also effective in expressing the ice nucleation activity in E. coli. Therefore, the resulting DNA fragment of approximately 5 kbp was considered to be an ina gene and named ina A. It existed as a unique gene in this strain of E. ananas. No corresponding ina gene existed in an ice nucleation-inactive strain of E. milletiae.

This publication has 0 references indexed in Scilit: