The effects of vitamin E and indomethacin on blood creatine Phosphokinase and fatty acid composition of tissues from young rabbits

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Abstract
The effects of vitamin E depletion and repletion and indomethacin treatment on blood creatine-phosphokinase (CPK) and fatty acid composition in phospholipids of the semitendinosus and gastrocnemius muscles, and in the heart, liver, kidney, and lung were studied in young rabbits. Male weanling New Zealand white rabbits were randomly divided into six treatments and fed a vitamin E-deficient diet. Treatment 1 was on the diet for 35 days; the second treatment was supplemented with 50 mg tocopherol acetate twice weekly; treatment 3 was on a deficient diet for 30 days and then supplemented with tocopherol acetate for another 30 days; the fourth treatment received vitamin E supplement for 60 days. Treatments 5 and 6 were the same as treatments 1 and 2, respectively, except for a daily addition of 25 mg indomethacin. Blood CPK was assayed in all treatments at 5-day intervals. Elevation of CPK was detected in the deficient animals as early as day 10 on the diet. Indomethacin-treated animals showed a consistently higher CPK values among all groups. Upon vitamin E refeeding, CPK value was lowered to the control level within 5 days. The phospholipids from both skeletal muscles showed significant decrease in 18-2 with a concurrent increase in 18-0, 20-3, and 20-4 in the vitamin E-deficient animals. The changes in the organs were less drastic. In the vitamin E-deficient, indomethacine-treated rabbits, fatty acid composition was similar to the E-deficient animals. After 30 days of vitamin E repletion, the changes in fatty acid composition had returned to the control values. The failure of indomethacin to prevent myopathy in the E-deficient rabbits suggests that either prostaglandin synthesis was not affected by the dosage used, or that prostaglandin synthesis is not directly involved in the etiology of nutritional muscular dystrophy.

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