Effect of positive redox potentials (>+400mV) on the expression of anaerobic respiratory enzymes in Escherichia coli
- 1 February 1990
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 4 (2) , 315-319
- https://doi.org/10.1111/j.1365-2958.1990.tb00598.x
Abstract
The expression of fumarate reductase and other enzymes of anaerobic respiration in Escherichia coli was studied as a function of the redox potential (E>h) in the medium. Redox potentials up to +300mV allowed full expression of fumarate reductase (frd) genes. Higher values resulted in decreased expression. The relationship between Eh and expression of frd could be approximated by the Nernst equation, assuming a redox couple with a midpoint potential E0′=+400mV to 440 mV. At Eh values >+510mV (generated anaerobically by hexacyanoferrate(III)) the degree of repression was the same as that obtained by O2. Hexacyanoferrate(III) also caused decreased activities of dimethylsulphoxide (DMSO), nitrite and nitrate reductases. Since expression of these enzymes depends on FNR, the gene activator of anaerobic respiratory genes, it is suggested that the function of FNR is controlled by a redox couple of E0′=+400mV to 440mV.This publication has 24 references indexed in Scilit:
- Inactivation of the FNR protein of Escherichia coli by targeted mutagenesis in the N‐terminal regionMolecular Microbiology, 1988
- Differential roles for menaquinone and demethylmenaquinone in anaerobic electron transport of E. coli and their fnr-independent expressionArchiv für Mikrobiologie, 1988
- On the role of cyclic AMP and the Fnr protein in Escherichia coli growing anaerobicallyArchiv für Mikrobiologie, 1987
- Isolation and characterization of the Fnr protein, the transcriptional regulator of anaerobic electron transport in Escherichia coliEuropean Journal of Biochemistry, 1985
- On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceaeArchiv für Mikrobiologie, 1983
- The orientation of the substrate sites of formate dehydrogenase and fumarate reductase in the membrane of Vibrio succinogenesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1980
- The Chromosomal Location and Pleiotropic Effects of Mutations of the nirA+ Gene of Escherichia coli k12: The Essential Role of nirA+ in Nitrite Reduction and in Other Anaerobic Redox ReactionsJournal of General Microbiology, 1978
- Mutants of Escherichia coli K12 Unable to use Fumarate as an Anaerobic Electron AcceptorJournal of General Microbiology, 1976
- The redox environment and microbial physiology. 1. The transition from anaerobiosis to aerobiosis in continuous cultures of facultative anaerobesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1971
- The effect of Eh on regulatory processes in facultative anaerobesBiotechnology & Bioengineering, 1969