THE ACTIVATION OF GLUTAMIC ACID AND GLUTAMINE IN MAMMALIAN TISSUES
- 1 May 1963
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 41 (5) , 1135-1145
- https://doi.org/10.1139/o63-129
Abstract
Measurements of α-glutamyl-RNA synthetase and glutaminyl-RNA synthetase activities have been made in fractions derived from 105,000 × g supernatants of homogenates of rat liver and of mouse Ehrlich ascites carcinoma cells. Evidence is presented which indicates that each amino acid is activated by a specific enzyme. Both enzymes catalyze amino acid dependent ATP-32PP exchange. The α-glutamyl-RNA synthetase purified 42-fold from rat liver 105,000 × g supernatant is free of glutamine synthetase.Keywords
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