A method to stabilize reduced and/or gas-treated protein crystals by flash-cooling under a controlled atmosphere

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Abstract
A customized glove box for protein crystallization under a controlled atmosphere is described along with a cryogenic technique adapted to freeze protein crystals inside the glove box and a very simple device for studying gas–protein complexes in the crystalline state at cryogenic temperatures. Using these techniques different redox states of oxygen-sensitive crystalline proteins have been stabilized and the interaction of hydrogenase with Xe, a model for the much lighter substrate molecular hydrogen, has been studied.

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