Co-adhesion of Oral Microbial Pairs under Flow in the Presence of Saliva and Lactose

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Abstract
Co-aggregation (interactions between two suspended micro-organisms) between oral microbial pairs has been studied extensively and is believed to be an important factor in dental plaque formation. However, coadhesion (interactions between suspended and already-adhering micro-organisms) may well be equally important. The aim of this paper was to determine the influence of saliva and lactose on the co-adhesion of streptococci (S. oralis 34 and S. sanguis PK1889) to actinomyces (A. naeslundii T14V-J1 or 5951) adhering on glass under flow from buffer and saliva in the absence and presence of lactose. The kinetics of co-adhesion as well as co-adhesion in a stationary end-point of co-aggregating and non-co-aggregating pairs was studied in a parallel plate flow chamber by analysis of the spatial arrangement of co-adhering micro-organisms as a function of time. For co-aggregating pairs, initial deposition rates of streptococci in the immediate vicinity of adhering actinomyces (local initial deposition rates) were up to 5 to 10 times higher than the non-local initial deposition rates in buffer and in saliva, respectively. In a stationary end-point of co-adhesion, 5 to 6 times more streptococci co-adhered with the adhering actinomyces than averaged over the entire substratum surface. A non-co-aggregating pair showed only minor preferential (co-)adhesion near the adhering actinomyces. Co-adhesion in buffer was fully lost when lactose was added. However, addition of lactose to saliva did not inhibit co-adhesion, but co-adhesion became more reversible. Detachment of micro-organisms from the substratum due to the passage of an air-liquid interface, as occurs in the oral cavity during eating, drinking, and speaking, was minimal when deposition was carried out from buffer to bare glass. Major detachment of streptococci adhering to the substratum occurred when adhesion was mediated through a salivary conditioning film on the glass, while detachment of adhering actinomyces and streptococci co-adhering with them remained low. It is suggested that, in the development of dental plaque, adhering actinomyces may act as strongholds for other micro-organisms, like streptococci, to adhere.