Changes in isoenzyme patterns during imbibition and germination of lodgepole pine (Pinuscontorta var. latifolia)

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Abstract
Isoenzymes of esterase (EST), glutamate-oxaloacetate transaminase (GOT), leucine aminopeptidase (LAP), and peroxidase (PER) were examined following various periods of imbibition and germination of lodgepole pine (Pinuscontorta var. latifolia Engelm.) seeds. The acidic and basic isoenzymes of embryos, and roots and shoots of germinating seedlings were analyzed by polyacrylamide gel electrophoresis. One major band of EST disappeared with imbibition, while some minor bands appeared and disappeared with imbibition and germination. Comparison of the roots and shoots after 7 and 14 days of germination showed several tissue-specific differences. The number of bands of GOT increased with imbibition and germination. The levels of activity of three isoenzymes differed between the root and shoot tissues. One band of LAP disappeared with imbibition. The levels of activity of two bands varied between the root and shoot tissues. The number of bands of PER increased dramatically following imbibition and germination. Also, many tissue-specific differences were observed between root and shoot tissues.

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