Evaluation of nonradioactive DNA probes for identification of mycobacteria
- 1 September 1992
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 30 (9) , 2476-2478
- https://doi.org/10.1128/jcm.30.9.2476-2478.1992
Abstract
Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and 22 Mycobacterium spp. The specificity was 100% for the four probes. The sensitivity was 100% for the MTB complex and M. gordonae probes and 95.2% for the MAC probe. Five of the nine M. kansasii isolates tested were not detected with the probe.Keywords
This publication has 8 references indexed in Scilit:
- Identification of Mycobacterium gordonae from culture by the Gen-Probe Rapid Diagnostic System: evaluation of 218 isolates and potential sources of false-negative resultsJournal of Clinical Microbiology, 1991
- Disseminated Mixed Mycobacterium simiae-Mycobacterium avium Complex Infection in Acquired Immunodeficiency SyndromeThe Journal of Infectious Diseases, 1991
- Assay formats involving acridinium-ester-labeled DNA probes.Clinical Chemistry, 1989
- Gen-probe test should not be considered final in Mycobacterium tuberculosis identificationJournal of Clinical Microbiology, 1989
- Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complexJournal of Clinical Microbiology, 1988
- Evaluation of Gen-Probe DNA hybridization systems for the identification of mycobacterium tuberculosis and mycobacterium avium-intracellulareDiagnostic Microbiology and Infectious Disease, 1987
- Mycobacterium simiae and Mycobacterium avium-M. intracellulare mixed infection in acquired immune deficiency syndromeJournal of Clinical Microbiology, 1987
- RAPID RADIOMETRIC METHODS TO DETECT AND DIFFERENTIATE MYCOBACTERIUM-TUBERCULOSIS M-BOVIS FROM OTHER MYCOBACTERIAL SPECIESPublished by Elsevier ,1984