Estrogen Receptors α and β
- 18 April 2000
- journal article
- other
- Published by Wolters Kluwer Health in Circulation
- Vol. 101 (15) , 1792-1798
- https://doi.org/10.1161/01.cir.101.15.1792
Abstract
Background —Estrogens have vascular effects through the activation of estrogen receptors (ERs). In addition to ERα, the first ER to be cloned, a second subtype called ERβ has recently been discovered. Methods and Results —Using a reverse-transcriptase polymerase chain reaction assay that employs the same primer pair to simultaneously amplify ERα and ERβ transcripts, we found that ERβ is the ER form that is predominantly expressed in human vascular smooth muscle, particularly in women. The transcriptional effects of the 2 ERs in transfected HeLa cells differed. In response to 17β-estradiol, ERα is a stronger transactivator than ERβ at low receptor concentrations. However, at higher receptor concentrations, ERα activity self-squelches, and ERβ is a stronger transactivator. Tamoxifen has partial agonist effects with ERα but not with ERβ. Conclusions —The protective effects of estrogens in the cardiovascular system of women may be due to the genomic effects of ERβ in vascular tissue.Keywords
This publication has 28 references indexed in Scilit:
- Differential Expression of Estrogen Receptor- (ER ) in Human Pituitary Tumors: Functional Interactions with ER and a Tumor-Specific Splice VariantJournal of Clinical Endocrinology & Metabolism, 1998
- Randomized Trial of Estrogen Plus Progestin for Secondary Prevention of Coronary Heart Disease in Postmenopausal WomenJAMA, 1998
- Different positioning of the ligand-binding domain helix 12and the F domain of the estrogen receptor accounts for functional differences between agonists and antagonistsThe EMBO Journal, 1998
- Differential Ligand Activation of Estrogen Receptors ERα and ERβ at AP1 SitesScience, 1997
- Agonistic Effect of Tamoxifen Is Dependent on Cell Type, ERE-Promoter Context, and Estrogen Receptor Subtype: Functional Difference between Estrogen Receptors α and βBiochemical and Biophysical Research Communications, 1997
- ERβ: Identification and characterization of a novel human estrogen receptorFEBS Letters, 1996
- Cloning of a novel receptor expressed in rat prostate and ovary.Proceedings of the National Academy of Sciences, 1996
- Identification of a Novel Isoform of Estrogen Receptor, a Potential Inhibitor of Estrogen Action, in Vascular Smooth Muscle CellsBiochemical and Biophysical Research Communications, 1996
- A third transactivation function (AF3) of human progesterone receptors located in the unique N-terminal segment of the B-isoformMolecular Endocrinology, 1994
- Estrogen replacement therapy and coronary heart disease: A quantitative assessment of the epidemiologic evidencePreventive Medicine, 1991