Biosynthetic labelling of the excretory and secretory antigens of Toxocara canis larvae

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Abstract
Toxocara canis larvae were cultured in vitro in medium containing [35S-]methionine for six days. The medium and the larval tissues were analysed for biosynthetically labelled polypcptides by sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography. Immunoprecipitates with positive and negative human antiserum were similarly analysed, using Staphylococcus aureus to absorb immunocom plexes. The larvae secrete biosynthctically labelled polypeptides into the medium, with three major polypcptides of molecular weights between 99 and 110 x 103 the major constituents. Both of these react strongly with human IgG in human positive scra. Many polypeptidcs become labelled in the larval tissue, but only one polypeptidc with similar molecular weight to the ES antigens, strongly reacted with human IgG.