Multicenter clinical comparison of resincontaining bottles with standard aerobic and Anaerobic bottles for culture of microorganisms from blood
- 1 September 1997
- journal article
- research article
- Published by Springer Nature in European Journal of Clinical Microbiology & Infectious Diseases
- Vol. 16 (9) , 669-674
- https://doi.org/10.1007/bf01708557
Abstract
In a study comparing the Bactec 9240 (Plus Aerobic/F and Anaerobic/F bottles, containing resins; Becton Dickinson, USA) and the Vital (standard aerobic and anaerobic bottles, with no additives; bioMérieux, France) blood culture systems, 6456 sets of four bottles of 9660 blood cultures submitted were evaluated. There were 531 clinically significant isolates from 795 positive blood cultures. Of the 531 positive blood cultures, 355 were positive in both systems, 141 with the Bactec 9240 alone, and 30 with the Vital alone (p < 0.001); five were not detected by either system. The average time to detection of positive cultures for the matched sets was 10.65 h and 18.41 h by the Bactec 9240 system and the Vital system, respectively. The false-positive rate per bottle was 0.65% in the Bactec 9240 and 0.71 % in the Vital. The rate of false-negative pairs (i.e., major errors) was very low (0.12% for the Bactec 9240, 0.19% for the Vital) and not significantly different between the two systems. The striking differences in recovery of microorganisms may be due to the presence of resins in the Bactec medium. However, the observed superiority of the Bactec 9240, even for patients not receiving antibiotics, suggests that resins adsorb other inhibitors present in patients' blood.Keywords
This publication has 18 references indexed in Scilit:
- Controlled clinical laboratory comparison of BACTEC plus aerobic/F resin medium with BacT/Alert aerobic FAN medium for detection of bacteremia and fungemiaJournal of Clinical Microbiology, 1997
- Evaluation of BACTEC 9240 blood culture system by using high-volume aerobic resin mediaJournal of Clinical Microbiology, 1995
- Detection kinetics for positive blood culture bottles by using the VITAL automated systemJournal of Clinical Microbiology, 1995
- Comparison of BACTEC 9240 and BacT/Alert blood culture systems in an adult hospitalJournal of Clinical Microbiology, 1995
- Value of terminal subcultures for blood cultures monitored by BACTEC 9240Journal of Clinical Microbiology, 1995
- Clinical evaluation of the Vital system compared with the Hémoline diphasic method for the detection of aerobic blood cultures.1995
- Multicenter clinical evaluation of a continuous monitoring blood culture system using fluorescent-sensor technology (BACTEC 9240)Journal of Clinical Microbiology, 1993