Autocrine Growth by Two Cytokines, Interleukin-6 and Tumor Necrosis Factor Alpha, in the Myeloma Cell Line KHM-1A
- 1 January 1990
- journal article
- research article
- Published by S. Karger AG in Acta Haematologica
- Vol. 83 (3) , 133-136
- https://doi.org/10.1159/000205189
Abstract
The effects of tumor necrosis factor .alpha. (TNF-.alpha.) and interleukin-6 (IL-6) on the cell growth of the myeloma cell line KHM-1A were studied. TNF-.alpha. strongly induced KHM-1A proliferation, while IL-6 weakly enhanced growth only at low cell densities. When TNF-.alpha. was combined with IL-6, TNF-.alpha.-induced growth enhancement was reduced. According to Northern blotting analysis, the m-RNA of both TNF-.alpha. and IL-6 were detected in KHM-1A. Moreover, monoclonal antibody capable of neutralizing the cytotoxic activity of TNF-.alpha. inhibited the proliferation of this cell line. These findings suggest that this cell line operates under an autocrine growth mechanism with respect to these two cytokines, IL-6 and TNF-.alpha.This publication has 14 references indexed in Scilit:
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