Stimulation of Cell-Free Protein Synthesis after Whole-Body Lethal X-Irradiation

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Abstract
Protein synthesis by liver microsomes in the presence of the appropriate cofactors, enzymes, and sRNA has been studied following 600 R of whole-body X-irradiation of rats. The synthesis, as measured by the uptake of L-leucine-U-C14 increased to a value about 70% greater than controls on day 9 postirradiation. During the overall period of observation (day 0 to day 15), the activity of the control systems increased 36%, while that of the experimental systems increased by 114%. When ribosomes and 105,000 x g supernatants from control and irradiated animals were interchanged, increased protein synthesis occurred whenever microsomes from irradiated animals were used. The degree of stimulation was shown to be a linear function of the relative amount of RNA in the microsomal fraction.