Quantitation and Estimation of Cooperation between Target‐Binding Sites in Natural and Antibody‐Dependent Cell‐Mediated Cytotoxicity by Use of a Mathematical Transformation

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Abstract
We have studied the use of a mathematical transformation, the Hill transformation, in natural and antibody‐dependent cell‐mediated cytotoxicity as a function of effector cell concentration. It can be concluded that when adherent cells are removed from the effector cell suspension, cooperation between binding sites for effector cells on the target cell changes from negative to zero. After stimulation of nonadherent cells with α‐interferon, the binding sites still remain noninteracting. This is the case both in natural and in antibody‐dependent cell‐mediated cytotoxicity experiments. The Hill transformation seems to be an easy and easily reproducible way of quantitating cytotoxicity.