IDENTIFICATION AND PARTIAL CHARACTERIZATION OF A MR 40,000 NUCLEOLAR ANTIGEN ASSOCIATED WITH CELL-PROLIFERATION
- 15 February 1987
- journal article
- research article
- Vol. 47 (4) , 1123-1129
Abstract
The present study reports the identification and partial characterization of a novel Mr 40,000 nucleolar antigen (P40) by monoclonal antibodies. Monoclonal antibodies to this protein were obtained when a nucleolar protein extract separated from the immunodominant protein C23 was used to immunize BALB/c mice; 12 hydridoma clones produced antibodies tothis protein. P40 was not detected in normal human kidney, liver, and leukocytes but was readily demonstrable in a variety of human malignant tissues. This newly identified P40 antigen differs in its specific nucleolar localization from cyclin (proliferating cell nuclear antigen), a Mr 35,000 antigen which is largely in the nucleoplasm. In addition, cyclin appears in the nucleolus in S-phase; P40 appears in the nucleolus 6 h after refeeding serum-starved HeLa cells.This publication has 20 references indexed in Scilit:
- A nuclear antigen associated with cell proliferation and blast transformation.The Journal of Experimental Medicine, 1981
- Nonhistone nuclear antigens reactive with autoantibodies. Immunofluorescence studies on distribution in synchronized cells.The Journal of cell biology, 1981
- Results of a preliminary “blind” study on the presence of the human tumor nucleolar antigen in breast carcinomas, benign breast tumors, and normal breast tissuesClinical Immunology and Immunopathology, 1981
- IDENTIFICATION AND PARTIAL-PURIFICATION OF HUMAN-TUMOR NUCLEOLAR ANTIGEN 54-6.31980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- NUCLEOLAR ANTIGEN FOUND IN A BROAD RANGE OF HUMAN-MALIGNANT TUMOR SPECIMENS1979
- DIFFERENCES IN NUCLEOLAR ANTIGENS OF RAT-LIVER AND NOVIKOFF HEPATOMA ASCITES-CELLS1978
- Antigenic Proteins of Nucleolar Chromatin of Novikoff Hepatoma Ascites CellsTumori Journal, 1977
- Visualization of ribosomal gene activity: Silver stains proteins associated with rRNA transcribed from oocyte chromosomesChromosoma, 1977
- A fetal protein in chromatin of Novikoff hepatoma and Walker 256 carcinosarcoma tumors that is absent from normal and regenerating rat liver.Proceedings of the National Academy of Sciences, 1976