Establishment of Hybridomas Secreting Monoclonal Antibodies Against Cε2 and Cε4 Domains of Human IgE

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Abstract
Three kind s of hybridomas secreting monoclonal antibodies (MAbs) against the ε chain of human IgE were constructed by somatic cell hybridization between mouse myeloma P3U1 cells and spleen cells from BALB/c mice immunized with human IgE purified from the culture supernatant of U-266 cells. These MAbs were used effectively for the purification and determination of human IgE. The recognition site in the IgE molecule of each antibody was examined by using various ε chain fragment peptides produced in Escherichia coli. From these experiments, it was suggested that one recognized Cε2 and the second Cε4. The third did not recognize the Cε1-Cε4 domains of the recombinant ε chain from E. coli, although it bound to the ε chain of natural human IgE.