Specific granules of the rat atrial muscle cell

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Abstract
The specific granules found in the atrial cardiac muscle cells of the normal rat were studied. The ultrastructural appearance of these granules demonstrates a fixative‐dependent lability. Fixation with cacodylate buffered aldehydes yields three types of granules. However, fixation with phosphate buffered aldehvdes or primary fixation with OsO4 yields granules of uniform appearance. The granules are found predominantly in the perinuclear zone; 78% of the granules are within ten linear micrometers of the center of the nucleus. Two independent methods of measurement demonstrate spherical diameters of these granules of 0.30 μm and 0.37 μ respectively. The granules are found in greater concentration at one pole of the nucleus than at the other. On the high density side there are 4.07 granules/μm3 which occupy 5.8% of the cytoplasmic volume. On the low density side there are 2.15 granules/μm3 which occupy 3.0% of the cytoplasmic volume. The granules at both poles are the same size. Atrial walls were incubated in a modified Tyrode's solution. One hour of incubation caused no change in the atrial granules. Addition of norepinephrine or L‐Dopa resulted in the appearance of more granules but the size of the granules remained the same. Incubation with reserpine had no effect upon the atrial granules. Apparently the atrial myocardial cell is stimulated by exogenous catechol‐amine to synthesize more atrial granules which themselves do not appear to contain catecholamines.
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