MONOSACCHARIDE COMPOSITION OF HUMAN MONOCLONAL (18) AND NORMAL (8) IGM-IMMUNOGLOBULINS - PROPOSED STRUCTURAL MODELS FOR THEIR GLYCAN CHAINS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 35  (4) , 133-136
Abstract
The carbohydrate composition of 18 monoclonal IgM samples from patients with Waldenstrom''s macroglobulinemia was determined by gas-liquid chromatography. Two populations occurred with mean sugar contents of 7.3% (12 IgM) and 10% (6 IgM). A value of 7.2% was obtained for 8 IgM prepared from 8 normal sera. On the basis of mean molar ratios established for each monosaccharide residue, structural models for oligosaccharide units are proposed. The number of complex glycan chains (N-acetyllactosaminic type) is higher in the 10% population, which corresponds to IgM with a mean sedimentation constant of 18.3 So20,W. The 7.3% population has a lower content of mature chains and its sedimentation constant is inferior: 17S020,W.