Neutralization of Cholera Enterotoxin-induced Steroidogenesis by Specific Antibody

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Abstract
A clonal line of adrenal cells in tissue culture responds to picogram quantities of cholera enterotoxin by undergoing morphologic changes and increasing its production of ketosteroids. When toxin is preincubated with antisera prepared against the purified enterotoxin or the naturally occurring toxoid, choleragenoid, both morphologic and steroidogenic changes are prevented. Partial, but not complete, neutralization of the effects of several crude preparations of toxigenic Escherichia coli was achieved when these toxin-containing preparations were incubated with antisera to cholera enterotoxin. This tissue culture system is a simple, reliable, relatively inexpensive, and sensitive (capable of detecting ⩽ 0.25 unit) assay system for the detection of cholera antitoxin.

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