Separation of Inhibitors of Hemagglutination and Specific Antibodies for Influenza Viruses by Starch Zone Electrophoresis

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Abstract
Summary: Specific antibodies, α inhibitor and β inhibitor against influenza viruses in serum were separated from each other by starch zone electrophoresis. Specific antibodies migrated only with γ globulin, β inhibitor was associated with the slowly moving β globulin, and α inhibitor usually migrated with α globulins. Antibodies in human serum and α inhibitor in human and rabbit serum each appeared to contain at least two components of different electrophoretic mobility and biological specificity. The α inhibitor activity of purified serum mucoprotein or acid glycoprotein was found to be due to a trace contaminant. The α inhibitor titer of human serum was elevated during the acute phase of various infectious diseases.