In vitro RNA editing in plant mitochondria does not require added energy
Open Access
- 21 May 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (14) , 2743-2747
- https://doi.org/10.1016/j.febslet.2007.05.025
Abstract
RNA editing in flowering plant mitochondria is investigated by in vitro assays. These cauliflower mitochondrial lysates require added NTP or dNTP. We have now resolved the reason for this requirement to be the inhibition of the RNA binding activity of the glutamate dehydrogenases (GDH). Both GDH1 and GDH2 were identified in RNA–protein cross‐links. The inhibition of in vitro RNA editing by GDH is confirmed by the ability of the GDH‐specific herbicide phosphinothricin to substitute for NTP. NADH and NADPH, but not NAD or NADP, can also replace NTP, suggesting that the NAD(P)H‐binding‐pocket configuration of the GDH contacts the RNA. RNA editing in plant mitochondria is thus intrinsically independent of added energy in the form of NTP.Keywords
This publication has 16 references indexed in Scilit:
- Partially edited RNAs are intermediates of RNA editing in plant mitochondriaThe Plant Journal, 2006
- RNA editing sites in plant mitochondria can share cis‐elementsFEBS Letters, 2005
- An in vitro RNA editing system from cauliflower mitochondria: Editing site recognition parameters can vary in different plant speciesRNA, 2005
- Substrate and cofactor requirements for RNA editing of chloroplast transcripts in Arabidopsis in vitroThe Plant Journal, 2005
- The plant mitochondrial proteomeTrends in Plant Science, 2005
- A site-specific factor interacts directly with its cognate RNA editing site in chloroplast transcriptsProceedings of the National Academy of Sciences, 2003
- In Vitro RNA Editing in Pea Mitochondria Requires NTP or dNTP, Suggesting Involvement of an RNA HelicaseJournal of Biological Chemistry, 2003
- Recognition of RNA Editing Sites Is Directed by Unique Proteins in Chloroplasts: Biochemical Identification ofcis-Acting Elements andtrans-Acting Factors Involved in RNA Editing in Tobacco and Pea ChloroplastsMolecular and Cellular Biology, 2002
- Arabidopsis mutant analysis and gene regulation define a nonredundant role for glutamate dehydrogenase in nitrogen assimilation.Proceedings of the National Academy of Sciences, 1996
- The mRNA‐binding protein COLBP is glutamate dehydrogenaseFEBS Letters, 1995