Generation of a velogenic Newcastle disease virus from cDNA and expression of the green fluorescent protein
- 29 March 2007
- journal article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 152 (7) , 1241-1249
- https://doi.org/10.1007/s00705-007-0961-x
Abstract
Newcastle disease virus (NDV) is a pathogen that is important in the poultry industry worldwide. Specifically, the virulent (velogenic) NDV is aparticular threat because it has now occurred frequently worldwide. The outbreaks caused by highly virulent NDV in waterfowl and especially in goose flocks, have led to greater concern in recent years as aquatic birds were previously resistant to most virulent NDV strains from chickens. The molecular determinants of host tropism, virulence and emergence of NDV isolated from diseased goose flocks are poorly understood. In the present study, we rescued a highly virulent NDV isolated from a goose using the reverse genetics approach. Infectious virus was successfully generated by cotransfection of a full-length cDNA clone of the NDV strain ZJ1 with helper plasmids. The recombinant NDV was indistinguishable from the parental wild-type virus with respect to its growth kinetics in cell culture as well as its biological properties. A recombinant NDV expressing green fluorescent protein (GFP) was generated, and GFP was subsequently detected in cells and various organs from the infected chickens.Keywords
This publication has 19 references indexed in Scilit:
- Engineered viral vaccine constructs with dual specificity: Avian influenza and Newcastle diseaseProceedings of the National Academy of Sciences, 2006
- Newcastle disease virus expressing H5 hemagglutinin gene protects chickens against Newcastle disease and avian influenzaProceedings of the National Academy of Sciences, 2006
- Selective gene transfer in vitro to tumor cells via recombinant Newcastle disease virusCancer Gene Therapy, 2004
- A Recombinant Newcastle Disease Virus (NDV) Expressing VP2 Protein of Infectious Bursal Disease Virus (IBDV) Protects against NDV and IBDVJournal of Virology, 2004
- Genomic sequence of an isolate of Newcastle disease virus isolated from an outbreak in geese: a novel six nucleotide insertion in the non-coding region of the nucleoprotein geneArchiv für die gesamte Virusforschung, 2004
- Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicityJournal of General Virology, 2003
- Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replicationJournal of General Virology, 2003
- A summary of taxonomic changes recently approved by ICTVArchiv für die gesamte Virusforschung, 2002
- Recombinant Newcastle Disease Virus as a Vaccine VectorJournal of Virology, 2001
- Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1)Avian Pathology, 2001