Decreased antioxidant defense during replicative aging of the yeastSaccharomyces cerevisiaestudied using the ‘baby machine’ method
- 7 March 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 492 (1-2) , 123-126
- https://doi.org/10.1016/s0014-5793(01)02244-x
Abstract
Replicatively senescent cells of Saccharomyces cerevisiae were obtained using the ‘baby machine’ method by immobilizing cells on CovaLink™ NH2 plates and allowing them to divide while exchanging medium and removing daughter cells. Centrifugation in a Percoll density gradient was employed for further purification of replicatively old yeast cells. Comparison of senescent cells showing more than 20 bud scars with cells from early stationary culture demonstrated a significant reduction of total and reduced glutathione and decrease of superoxide dismutase activity during replicative aging of yeast cells.Keywords
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