Spinning-disk Confocal Microscopy. A Cutting-Edge Tool for Imaging of Membrane Traffic.
- 1 January 2002
- journal article
- review article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 27 (5) , 349-355
- https://doi.org/10.1247/csf.27.349
Abstract
Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.Keywords
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