Determination of susceptibility of Staphylococcus aureus to methicillin by luciferim‐luciferase assay of bacterial adenosine triphosphate

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Abstract
Susceptibility of 50 strains of S. aureus to methicillin was determined by disc diffusion, minimum inhibitory concentration (MIC) test and luciferin-luciferase assay of bacterial ATP. ATP concentration-time curves calculated on 8 strains (4 sensitive, 4 resistant) incubated at 30.degree. C indicated 2.5 h as the optimum time for determination of methicillin susceptibility. The ATP concentration and relative light unit (RLU) value of each test broth (antibiotic-containing), expressed as a percentage of its own control broth (antibiotic-free), indicated values of < 30% for ATP and < 40% for RLU to be indicative of methicillin sensitivity. Single time point ATP assays carried out on the 50 strains after 2.5 h at 30.degree. C correlated exactly with disc diffusion and MIC. Luciferin-luciferase assay of bacterial ATP appeared to be a reliable, rapid technique for determining the susceptibility of S. aureus to methicillin.

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