Na+, K+‐specific inhibition of protein and peptide hydrolyses by proteasomes from human hepatoma tissues
Open Access
- 24 April 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 247 (2) , 197-200
- https://doi.org/10.1016/0014-5793(89)81333-x
Abstract
Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine‐activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, although to a lesser extent. On the other hand, they reversed the inhibitory and stimulatory effects of polylysine on the hydrolyses of Suc‐Leu‐Tyr‐AMC and Cbz‐Ala‐Arg‐Arg‐MNA, respectively. These results suggest that Na+ and/or K+ may be involved in the regulation of intracellular protein breakdown by controlling the multicatalytic activity of proteasomes.Keywords
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