Influence of Some Nonionic Surfactants on Pancreatic Lipase Activity

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Abstract
Summary Two nonionic surfactants, Triton WR-1339 and Macrocyclon inhibit the hydrolysis of triglycerides in a coconut oil emulsion (Ediol) by pancreatic lipase, Triton inhibiting to a greater extent than Macrocyclon in a ratio of approximately 2:1. HOC-60, also a nonionic surfactant, is not inhibitory. The results of the present study indicate that during its incubation with the surfactant at 37°;C for 1 hour before adding the enzyme, the substrate may undergo a change which would result in an altered substrate molecule that is less reactive to pancreatic lipase. Two suggested mechanisms of the inhibitory action of the nonionic surfactants are discussed; (1) formation of an irreversible complex and (2) formation of a micelle. The ability of these agents to induce hyperlipemia in animals was reflected by inhibition of enzymatic lipolysis in vitro.

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