G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression
Open Access
- 15 March 2003
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 17 (6) , 723-728
- https://doi.org/10.1101/gad.1031803
Abstract
Here we show that the cell cycle defects of dE2F1-depleted cells depend on the cooperative effects of dE2F2 and DACAPO (DAP), an inhibitor of Cyclin E/cyclin-dependent kinase 2 (CycE/cdk2). The different properties of cells lacking dE2F1/dE2F2 and dE2F1/DAP lead to the surprising observation that dE2F2-mediated repression differs from retinoblastoma family protein 1 (RBF1) inhibition of dE2F1, and is resistant to both CycE/cdk2 and Cyclin D/cyclin-dependent kinase 4 (CycD/cdk4). This resistance occurs even though dE2F2/RBF1 complexes are disrupted by CycE/cdk2, and may explain why dE2F2 is so potent in the absence of de2f1. The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression.Keywords
This publication has 27 references indexed in Scilit:
- A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G 0 CellsScience, 2002
- E2F mediates cell cycle-dependent transcriptional repression in vivo by recruitment of an HDAC1/mSin3B corepressor complexGenes & Development, 2002
- Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysisGenes & Development, 2002
- Sibling rivalry in the E2F familyNature Reviews Molecular Cell Biology, 2002
- The E2F1–3 transcription factors are essential for cellular proliferationNature, 2001
- Functional antagonism between E2F family membersGenes & Development, 2001
- dE2F2, a Novel E2F-Family Transcription Factor inDrosophila melanogasterBiochemical and Biophysical Research Communications, 1998
- Cyclin E-induced S phase without activation of the pRb/E2F pathway.Genes & Development, 1997
- RBF, a novel RB-related gene that regulates E2F activity and interacts with cyclin E in Drosophila.Genes & Development, 1996
- Cell Cycle Regulation of E2F Site Occupation in VivoScience, 1996